期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 110, 期 7, 页码 1811-1821出版社
WILEY
DOI: 10.1002/bit.24890
关键词
double-strand break; genome engineering; gene targeting; homologous recombination; non-homologous end joining
资金
- National Institutes of Health [R21 HL089418]
- Department of Chemical and Biomolecular Engineering at the University of Illinois at Urbana-Champaign
Transcription activator-like effector (TALE) nucleases (TALENs) have recently emerged as a revolutionary genome editing tool in many different organisms and cell types. The site-specific chromosomal double-strand breaks introduced by TALENs significantly increase the efficiency of genomic modification. The modular nature of the TALE central repeat domains enables researchers to tailor DNA recognition specificity with ease and target essentially any desired DNA sequence. Here, we comprehensively review the development of TALEN technology in terms of scaffold optimization, DNA recognition, and repeat array assembly. In addition, we provide some perspectives on the future development of this technology. Biotechnol. Bioeng. 2013; 110: 1811-1821. (c) 2013 Wiley Periodicals, Inc.
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