期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 110, 期 7, 页码 1895-1902出版社
WILEY
DOI: 10.1002/bit.24862
关键词
cholinium amino acids ionic liquids; delignification; enzymatic hydrolysis; lignin distribution; lignocellulosic biomass; pretreatment
资金
- National Natural Science Foundation of China [20906032, 21072065]
- Research Funds for Zhujiang Nova Program [2012J2200011]
- Fundamental Research Funds for the Central Universities, SCUT [2012ZP0009]
Pretreatment of rice straw by using renewable cholinium amino acids ionic liquids ([Ch][AA] ILs)-water mixtures and the subsequent enzymatic hydrolysis of the residues were conducted in the present work. Of the eight mixtures composed of ILs and water, most were found to be effective for rice straw pretreatment. After pretreatment with 50% ILs-water mixtures, the enzymatic digestion of the lignocellulosic biomass was enhanced significantly, thus leading to satisfactory sugar yields of >80% for glucose and approximately 50% for xylose. To better understand the ILs pretreatment mechanism, confocal laser scanning microscopy combined with immunolabeling and transmission electron microscopy were used to visualize changes in the contents and distribution of two major componentslignin and xylan. The results coupled with changes in chemical structures (infrared spectra) of the substrates indicated occurrence of extensive delignification, especially in cell corner and compound middle lumen of cell walls, which made polysaccharides more accessible to enzymes. This pretreatment process is promising for large-scale application because of the high sugar yields, easy handling, being environmentally benign and highly tolerant to moisture, and significantly reduced cost and energy consumption. Biotechnol. Bioeng. 2013; 110: 1895-1902. (c) 2013 Wiley Periodicals, Inc.
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