期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 110, 期 5, 页码 1302-1311出版社
WILEY-BLACKWELL
DOI: 10.1002/bit.24797
关键词
continuous fermentation; SSCF; SHF; cellulosic ethanol; AFEX; enzymatic hydrolysis
资金
- U.S. Department of Energy through the DOE Great Lakes Bioenergy Research Center (GLBRC) [DE-FC02-07ER64494]
High productivity processes are critical for commercial production of cellulosic ethanol. One high productivity processcontinuous hydrolysis and fermentationhas been applied in corn ethanol industry. However, little research related to this process has been conducted on cellulosic ethanol production. Here, we report and compare the kinetics of both batch SHF (separate hydrolysis and co-fermentation) and SSCF (simultaneous saccharification and co-fermentation) of AFEX (Ammonia Fiber Expansion) pretreated corn stover (AFEX-CS). Subsequently, we designed a SSCF process to evaluate continuous hydrolysis and fermentation performance on AFEX-CS in a series of continuous stirred tank reactors (CSTRs). Based on similar sugar to ethanol conversions (around 80% glucose-to-ethanol conversion and 47% xylose-to-ethanol conversion), the overall process ethanol productivity for continuous SSCF was 2.3- and 1.8-fold higher than batch SHF and SSCF, respectively. Slow xylose fermentation and high concentrations of xylose oligomers were the major factors limiting further enhancement of productivity. Biotechnol. Bioeng. 2013; 110: 13021311. (c) 2012 Wiley Periodicals, Inc.
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