期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 109, 期 1, 页码 92-99出版社
WILEY
DOI: 10.1002/bit.23296
关键词
ss-glucosidase; Trichoderma reesei; xyn3; Aspergillus aculeatus; cellulase; bioconversion
资金
- New Energy and Industrial Technology Development Organization (NEDO)
- Grants-in-Aid for Scientific Research [22580090] Funding Source: KAKEN
To develop a Trichoderma reesei strain appropriate for the saccharification of pretreated cellulosic biomass, a recombinant T. reesei strain, X3AB1, was constructed that expressed an Aspergillus aculeatus beta-glucosidase 1 with high specific activity under the control of the xyn3 promoter. The culture supernatant from T. reesei X3AB1 grown on 1% Avicel as a carbon source had 63- and 25-fold higher beta-glucosidase activity against cellobiose compared to that of the parent strain PC-3-7 and that of the T. reesei recombinant strain expressing an endogenous beta-glucosidase I, respectively. Further, the xylanase activity was 30% lower than that of PC-3-7 due to the absence of xyn3. X3AB1 grown on 1% Avicel-0.5% xylan medium produced 2.3- and 3.3-fold more xylanase and beta-xylosidase, respectively, than X3AB1 grown on 1% Avicel. The supernatant from X3AB1 grown on Avicel and xylan saccharified NaOH-pretreated rice straw efficiently at a low enzyme dose, indicating that the strain has good potential for use in cellulosic biomass conversion processes. Biotechnol. Bioeng. 2012;109: 9299. (c) 2011 Wiley Periodicals, Inc.
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