期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 108, 期 7, 页码 1549-1558出版社
WILEY
DOI: 10.1002/bit.23090
关键词
organosolv; cellulase; enzymatic hydrolysis; ethanol; lignin; sulfonation; refining
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
Organosolv-pretreated Lodgepole pine substrates were physically and chemically treated to increase their hydrophilicity and swelling as these are two substrate attributes which have been shown to improve cellulolytic hydrolysis. Surprisingly, mechanical treatment of the organosolv-treated substrates by PFI-mill refining did not significantly increase hydrolysis yields despite decreases in particle size and crystallinity and increases in swelling. However, sulfonation of the substrate did, significantly, increase enzymatic hydrolysis at loadings of both 5 and 2.5 FPU g(-1) cellulose (from 80% to 95% and from 35% to 80%, respectively). In addition, sulfonation resulted in an increase in the amount of free enzymes detected during the course of hydrolysis to a maximum of 80% after 72 h. This suggested that the beneficial effects of sulfonation were primarily due to a decrease in the non-specific binding of the cellulases to the lignin. Biotechnol. Bioeng. 2011; 108: 1549-1558. (C) 2011 Wiley Periodicals, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据