期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 109, 期 4, 页码 1095-1099出版社
WILEY-BLACKWELL
DOI: 10.1002/bit.24368
关键词
flow perfusion bioreactor; tissue engineering; mathematical modeling; computational fluid dynamics (CFD); porous medium flow; wall shear stress; rectangular porous scaffold
资金
- Biotechnology and Biological Sciences Research Council (BBSRC) [BB/F013892/1]
- Engineering and Physical Sciences Research Council (EPSRC) Bridging the Gap:3ME Initiative-Keele University
- Biotechnology and Biological Sciences Research Council [BB/F013892/1, BB/F013892/2] Funding Source: researchfish
- BBSRC [BB/F013892/1, BB/F013892/2] Funding Source: UKRI
Perfusion bioreactors have been used in different tissue engineering applications because of their consistent distribution of nutrients and flow-induced shear stress within the tissue-engineering scaffold. A widely used configuration uses a scaffold with a circular cross-section enclosed within a cylindrical chamber and inlet and outlet pipes which are connected to the chamber on either side through which media is continuously circulated. However, fluid-flow experiments and simulations have shown that the majority of the flow perfuses through the center. This pattern creates stagnant zones in the peripheral regions as well as in those of high flow rate near the inlet and outlet. This non-uniformity of flow and shear stress, owing to a circular design, results in limited cell proliferation and differentiation in these areas. The focus of this communication is to design an optimized perfusion system using computational fluid dynamics as a mathematical tool to overcome the time-consuming trial and error experimental method. We compared the flow within a circular and a rectangular bioreactor system. Flow simulations within the rectangular bioreactor are shown to overcome the limitations in the circular design. This communication challenges the circular cross-section bioreactor configuration paradigm and provides proof of the advantages of the new design over the existing one. Biotechnol. Bioeng. 2012; 109:10951099. (c) 2011 Wiley Periodicals, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据