期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 107, 期 2, 页码 195-205出版社
WILEY
DOI: 10.1002/bit.22838
关键词
glycoside hydrolases; catalytic mechanism; catalytic base; nucleophile; diversity
资金
- DOE Office of Biological and Environmental Research
Recent studies have shown that a number of glycoside hydrolase families do not follow the classical catalytic mechanisms, as they lack a typical catalytic base/nucleophile. A variety of mechanisms are used to replace this function, including substrate-assisted catalysis, a network of several residues, and the use of non-carboxylate residues or exogenous nucleophiles. Removal of the catalytic base/nucleophile by mutation can have a profound impact on substrate specificity, producing enzymes with completely new functions. Biotechnol. Bioeng. 2010;107: 195-205. (c) 2010 Wiley Periodicals, Inc.
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