期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 108, 期 1, 页码 31-40出版社
WILEY
DOI: 10.1002/bit.22927
关键词
Clostridium tyrobutyricum; butyric acid fermentation; fibrous bed bioreactor; immobilization; adaptation
资金
- National High Technology Research and Development Program of China [2009AA02Z206]
- National Basic Research Program of China [2009CB724700]
- Ministry of Science and Technology, China
- National Natural Science Foundation of China [20936002]
Repeated fed-batch fermentation of glucose by Clostridium tyrobutyricum immobilized in a fibrous bed bioreactor (FBB) was successfully employed to produce butyric acid at a high final concentration as well as to adapt a butyric-acid-tolerant strain. At the end of the eighth fed-batch fermentation, the butyric acid concentration reached 86.9 +/- 2.17 g/L, which to our knowledge is the highest butyric acid concentration ever produced in the traditional fermentation process. To understand the mechanism and factors contributing to the improved butyric acid production and enhanced acid tolerance, adapted strains were harvested from the FBB and characterized for their physiological properties, including specific growth rate, acid-forming enzymes, intracellular pH, membrane-bound ATPase and cell morphology. Compared with the original culture used to seed the bioreactor, the adapted culture showed significantly reduced inhibition effects of butyric acid on specific growth rate, cellular activities of butyric-acid-forming enzyme phosphotransbutyrylase (PTB) and ATPase, together with elevated intracellular pH, and elongated rod morphology. Biotechnol. Bioeng. 2011;108: 31-40. (C) 2010 Wiley Periodicals, Inc.
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