Carotenoid and Fatty Acid Metabolism in Light-Stressed Dunaliella salina

beta-Carotene is overproduced in the alga Dunaliella salina in response to high light intensities. We have studied the effects of a sudden light increase on carotenoid and fatty acid metabolism using a flat panel photobioreactor that was run in turbidostat mode to ensure a constant light regime throughout the experiments. Upon the shift to an increased light intensity, beta-carotene production commenced immediately. The first 4h after induction were marked by constant intracellular levels of beta-carotene (2.2 g LCV-1), which resulted from identical increases in the production rates of cell volume and beta-carotene. Following this initial phase, beta-carotene productivity continued to increase while the cell volume productivity dropped. As a result, the intracellular beta-carotene concentration increased reaching a maximum of 17 g LCV-1 after 2 days of light stress. Approximately 1 day before that, the maximum beta-carotene productivity of 30 pg cell(-1) day(-1) (equivalent to 37 mg LRV-1 day(-1)) was obtained, which was about one order of magnitude larger than the average productivity reported for a commercial beta-carotene production facility indicating a vast potential for improvement Furthermore, by studying the light-induced changes in both beta-carotene and fatty acid metabolism, it appeared that carotenoid overproduction was associated with oil globule formation and a decrease in the degree of fatty acid unsaturation Our results indicate that cellular beta-carotene accumulation in D salina correlates with accumulation of specific fatty acid species (C16 0 and C18 1) rather than with total fatty acid content. Biotechnol. Bioeng. 2010,106: 638-648, (C) 2010 Wiley Periodicals, Inc.