期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1393, 期 -, 页码 115-121出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2015.03.025
关键词
Asymmetrical flow field-flow fractionation; Channel thickness determination; Sample-membrane interaction; Steric effect
资金
- National Research Foundation (NRF) of Korea [NRF-2014M2A8A5021929]
Asymmetrical flow field-flow fractionation (AF4) has been considered to be a useful tool for simultaneous separation and characterization of polydisperse macromolecules or colloidal nanoparticles. AF4 analysis requires the knowledge of the channel thickness (w), which is usually measured by injecting a standard with known diffusion coefficient (D) or hydrodynamic diameter (d(h)). An accurate w determination is a challenge due to its uncertainties arising from the membrane's compressibility, which may vary with experimental condition. In the present study, influence of factors including the size and type of the standard on the measurement of w was systematically investigated. The results revealed that steric effect and the particles membrane interaction by van der Waals or electrostatic force may result in an error in w measurement. (C) 2015 Elsevier B.V. All rights reserved.
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