Syntrophic Interactions Among Anode Respiring Bacteria (ARB) and Non-ARB in a Biofilm Anode: Electron Balances

We demonstrate that the coulombic efficiency (CE) of a microbial electrolytic cell (MEC) fueled with a fermentable substrate, ethanol, depended on the interactions among anode respiring bacteria (ARB) and other groups Of micro-organisms, particularly fermenters and methanogens. When we allowed methanogenesis, we obtained a CE of 60%, and 26% of the electrons were lost as methane. The only methanogenic genus detected by quantitative real-time PCR was the hydrogenotrophic genus, Methanobacteriales, which presumably consumed all the hydrogen produced during ethanol fermentation (similar to 30% of total electrons). We did not detect acetoclastic methanogenic genera, indicating that acetate-oxidizing ARB out-competed acetoclastic methanogens. Current production and methane formation increased in parallel, suggesting a syntrophic interaction between methanogens and acetate-consuming ARB. When we inhibited methanogenesis with 50 mM 2-bromoethane sulfonic acid (BES), the CE increased to 84%, and methane was not produced. With no methanogenesis, the electrons from hydrogen were converted to electrical Current, either directly by the ARB or channeled to acetate through homo-acetogenesis. This illustrates the key role of competition among the various H, scavengers and that, when the hydrogen-consuming methanogens were present, they out-competed the other groups. These findings also demonstrate the importance of a three-way syntrophic relationship among fermenters, acetate-consuming ARB, and a H, consumer during the utilization of a fermentable substrate. To obtain high coulombic efficiencies with fermentable substrates in a mixed population, methanogens must be Suppressed to promote new interactions at the anode that ultimately channel the electrons from hydrogen to Current. Biotechnol. Bioeng. 2009;103: 513-523. (C) 2009 Wiley Periodicals, Inc.