期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 102, 期 4, 页码 1161-1169出版社
WILEY
DOI: 10.1002/bit.22129
关键词
lactate; exponential growth; metabotypes; phylotypes; flux profiles
资金
- U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research, Genomics:GTL Program [DE-AC02-05CH11231]
- Lawrence Berkeley National Laboratory
- US Department of Energy
- Joint BioEnergy Institute
Shewanella spp. are a group of facultative anaerobic bacteria widely distributed in marine and freshwater environments. In this Study, we profiled the central metabolic fluxes of eight recently sequenced Shewanella species grown under the same condition in minimal medium with [3-C-13] lactate. Although the tested Shewanella species had slightly different growth rates (0.23-0.29 h(-1)) and produced different amounts of acetate and pyruvate during early exponential growth (pseudo-steady state), the relative intracellular metabolic flux distributions were remarkably similar. This result indicates that Shewanella species share similar regulation in regard to central carbon metabolic fluxes under steady growth conditions: the maintenance of metabolic robustness is not only evident in a single species under genetic perturbations (Fischer and Sauer, 2005; Nat Genet 37(6):636-640), but also observed through evolutionary related microbial species. This remarkable conservation of relative flux profiles through phylogenetic differences prompts LIS to introduce the concept of metabotype as an alternative scheme to classify microbial fluxomics. On the other hand, Shewanella spp. display flexibility in the relative flux profiles when switching their metabolism from consuming lactate to consuming pyruvate and acetate.
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