期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 102, 期 6, 页码 1636-1644出版社
WILEY-BLACKWELL
DOI: 10.1002/bit.22187
关键词
human embryonic stem cells; automation; CompacT SelecT; scalability; process control
资金
- HUES-7
- MRC
- BBSRC
- EPSRC
- University of Nottingham
- UK RC Fellowship
- Biotechnology and Biological Sciences Research Council [BB/E006159/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [GR/S44518/01, EP/C534247/1] Funding Source: researchfish
- Medical Research Council [G113/30] Funding Source: researchfish
- BBSRC [BB/E006159/1] Funding Source: UKRI
- MRC [G113/30] Funding Source: UKRI
Large-scale manufacture of human embryonic stem cells (hESCs) is prerequisite to their widespread use in biomedical applications. However, current hESC culture strategies are labor-intensive and employ highly variable processes, presenting challenges for scaled production and commercial development. Here we demonstrate that passaging of the hESC lines, HUES7, and NOTT1, with trypsin in feeder-free conditions, is compatible with complete automation on the CompacT SelecT, a commercially available and industrially relevant robotic platform. Pluripotency was successfully retained, as evidenced by consistent proliferation during serial passage, expression of stem cell markers (OCT4, NANOG, TRA1-81, and SSEA-4), stable karyotype, and multi-germlayer differentiation in vitro, including to pharmacologically responsive cardiomyocytes. Automation of hESC Culture will expedite cell-use in clinical, scientific, and industrial applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据