期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 103, 期 2, 页码 241-251出版社
WILEY
DOI: 10.1002/bit.22274
关键词
self-assembling peptide; nanostructured material; recombinant peptide
资金
- BBSRC
- Dow Benelux BV
- University of Leeds
- Royal Society
Peptide P-II-4 (QQRFEWEFEQQ) was designed to self-assemble to form beta-sheets and nematic gels in the pH range 5-7 at concentrations >= 12.6 mM in water. This self-assembly is reversibly controlled by adjusting the pH of the solvent. It can also self-assemble into gels in biological media. This together with its biocompatibility and biodegradability make P-II-4 an attractive building block for the fabrication of nanoscale materials with uses in, for example, tissue engineering. A limitation to large-scale production of such peptides is the high cost of solid phase chemical synthesis. We describe expression of peptide P-II-4 in the bacterium Escherichia coli from constructs carrying tandem repeats of the peptide coding sequence. The vector pET31b+ Was used to express P-II-4 repeats fused to the ketosteroid isomerase protein which accumulates in easily recoverable inclusion bodies. Importantly, the use of auto-induction growth medium to enhance cell density and protein expression levels resulted in recovery of 2.5 g fusion protein/L culture in both shake flask and batch fermentation. Whole cell detergent lysis allowed recovery of inclusion bodies largely composed of the fusion protein. Cyanogen bromide cleavage followed by reverse phase HPLC allowed purification of the recombinant peptide with a C-terminal homoserine lactone (rP(II)-4(hsl)). This recombinant peptide formed pH dependent hydrogels, displayed P-structure measured by circular dichroism and fibril formation observed by, transmission electron microscopy. Biotechnol. Bioeng. 2009;103: 241-251. (C) 2009 Wiley Periodicals, Inc.
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