期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 105, 期 4, 页码 834-841出版社
WILEY
DOI: 10.1002/bit.22583
关键词
electrohydrodynamic spraying; coaxial electrospray; plasmid DNA; polyethylenimine; polyplex; nonviral gene delivery
资金
- National Science Foundation [EEC-0425626]
DNA/polyethylenimine (PEI) polyplexes are an important class of nonviral vectors. Although the conventional preparation method, bulk mixing, is straightforward, the formation of the DNA/PEI polyplexes is not well controlled. This work explores coaxial electrohydrodynamic spraying (EHDS) as a novel, alternative method to produce DNA/PEI polyplexes in a more controlled manner. Both pGFP/PEI and pSEAP/PEI polyplexes were produced by EHDS with a coaxial needle setup. The size of the polyplexes was determined using dynamic light scattering, and their ability to transfect NIH 3T3 cells was observed by using an inverted fluorescence microscope (pGFP) or quantified by measuring the activity level of alkaline phosphatase (pSEAP). At nitrogen to phosphate ratio (N/P) of 6.7, the polyplexes produced by coaxial EHDS had delivery efficiencies up to 2.6 times higher than those produced by bulk mixing. The N/P ratio and the structure of the EHDS used to make the polyplexes were crucial factors in determining the delivery efficiency. Biotechnol. Bioeng. 2010;105: 834-841. (C) 2009 Wiley Periodicals, Inc.
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