期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 103, 期 1, 页码 207-216出版社
JOHN WILEY & SONS INC
DOI: 10.1002/bit.22218
关键词
exopolysaccharides (EPS); Halomonas; anionic residues; methylene blue; emulsification; hexadecane
资金
- Natural Environment Research Council [NE/E523272/1]
- NERC [NE/E523272/1] Funding Source: UKRI
- Natural Environment Research Council [NE/E523272/1] Funding Source: researchfish
In this Study, We investigated the yield and physicochemical properties of the high molecular weight extracellular polymeric substance (HMW-EPS) produced by Halomonas sp. strain TG39 When grown on different types and ratios of substrates, Glucose (1% w/v) and a peptone/yeast extract ratio of 5.1 (0.6% w/v final concentration) yielded an EPS fraction (HMW-glucose) exhibiting the highest anionic activity (20.5) and specific emulsifying activity (EI(24) = 100%) compared to EPS produced by cells grown on mannitol, sucrose, malt extract or no carbon source. The HMW-EPS fractions were capable of binding approximate to 255-464 mg of methylene blue (MB) per gram of EPS, which represents the highest reported binding of MB by a bacterial EPS. A comparative evaluation of these properties to those of commercial hydrocolloids indicated that the combined effect of protein and anionic residues of the HMW-EPS contributed to its ability to emulsify n-hexadecane. Liquid chromatography revealed the HMW-glucose EPS to be a heterogeneous polymer with a polydispersity index of 1.8. This work presents evidence of a correlation between the anionic nature and protein content of bacterial EPS with its emulsifying qualities, and identifies EPS produced by strain TG39 as a high MB-binding bacterial sorbant With potential biotechnological application.
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