期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 103, 期 2, 页码 323-328出版社
WILEY
DOI: 10.1002/bit.22255
关键词
ultrasonic manipulation; cell characterization; microfluidic chip
资金
- European Community [NMP4-CT-2004-500039]
- Swedish Research Council for Engineering Sciences
- Goran Gustavsson Foundation
We demonstrate selective retention and positioning of cells or other bioparticles by ultrasonic manipulation in a microfluidic expansion chamber during microfluidic perfusion. The chamber is designed as a confocal ultrasonic resonator for maximum confinement of the ultrasonic force field at the chamber center, where the cells are trapped. We investigate the resonant modes in the expansion chamber and its connecting inlet channel by theoretical modeling and experimental verification during no-flow conditions. Furthermore, by triple-frequency ultrasonic actuation during continuous microfluidic sample feeding, a set of several manipulation functions performed in series is demonstrated: sample bypass-injection-aggregation and retention-positioning. Finally, we demonstrate transillumination microscopy imaging Of Ultrasonically trapped COS-7 cell aggregates. Biotechnol. Bioeng. 2009;103: 323-328. (C) 2009 Wiley Periodicals, Inc.
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