4.6 Article

Monolayer cell expansion conditions affect the chondrogenic potential of adipose-derived stem cells

期刊

BIOTECHNOLOGY AND BIOENGINEERING
卷 99, 期 4, 页码 986-995

出版社

WILEY
DOI: 10.1002/bit.21662

关键词

adult stem cells; differentiation; mesenchymal stem cells; adipose stem cells; progenitor cells; chondrogenesis

资金

  1. NCRR NIH HHS [UL1 RR024128, UL1RR024128] Funding Source: Medline
  2. NIAMS NIH HHS [P01 AR050245, AR50245, AR48182, R01 AR048182, R01 AR048852] Funding Source: Medline
  3. NIA NIH HHS [AG15768, R01 AG015768] Funding Source: Medline
  4. NIGMS NIH HHS [GM08555, T32 GM008555] Funding Source: Medline

向作者/读者索取更多资源

Adipose-derived stem cells (ASCs) are an abundant, readily available population of multipotent progenitor cells that reside in adipose tissue. Isolated ASCs are typically expanded in monolayer on standard tissue culture plastic with a basal medium containing 10% fetal bovine serum. However, recent data suggest that altering the monolayer expansion conditions by using suspension culture plastic, adding growth factors to the medium, or adjusting the seeding density may affect the self-renewal rate, multipotency, and lineage-specific differentiation potential of the ASCs. We hypothesized that variation in any of these expansion conditions would influence the chondrogenic potential of ASCs. ASCs were isolated from human liposuction waste tissue and expanded through two passages with different tissue culture plastic, feed medium, and cell seeding densities. Once expanded, the cells were cast in an agarose gel and subjected to identical chondrogenic culture conditions for 7 days, at which point cell viability, radiolabel incorporation, and gene expression were measured. High rates of matrix synthesis upon chondrogenic induction were mostly associated with smaller cells, as indicated by cell width and area on tissue culture plastic, and it appears that expansion in a growth factor supplemented medium is important in maintaining this morphology. All end-point measures were highly dependent on the specific monolayer culture conditions. These results support the hypothesis that monolayer culture conditions may prime the cells or predispose them towards a specific phenotype and thus underscore the importance of early culture conditions in determining the growth and differentiation potential of ASCs..

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据