期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 101, 期 1, 页码 1-8出版社
WILEY
DOI: 10.1002/bit.21874
关键词
nanomanipulation; MicroTAS; MEMS; nanotransport; biomimetics; kinesin; dynein
资金
- Ministry of Education, Culture, Sports, Science and Technology, (MEXT), Japan
- Young Scientists (B) [19710111]
- School of Materials Science
- Japan Advanced Institute of Science and Technology (JAIST)
- Department of Bioscience and Biotechnology, Ritsumcikan University
- Institute of Industrial Science, The University of Tokyo
Artificial nanotransport systems inspired by intracellular transport processes have been investigated for over a decade using the motor protein kinesin and microtubules. However, Only unidirectional cargo transport has been achieved for the purpose of nanotransport in a microfluidic system. Here, we demonstrate bidirectional nanotransport by integrating kinesin and dynein motor proteins. Our molecular system allows microtubule orientation of either polarity in a microfluidic channel to construct a transport track. Each motor protein acts as a nanoactuators that transports microspheres in opposite directions determined by the polarity of the oriented microtubules: kinesin-coated microspheres move toward the plus end of microtubules, whereas dynein-coated microspheres move toward the minus end. We demonstrate both unidirectional and bidirectional transport using kinesin- and dyneincoated microspheres on microtubules oriented and glutaraldehyde-immobilized in a microfluidic channel. Tracking and statistical analysis of microsphere movement demonstrate that 87-98% of microspheres move in the designated direction at a mean velocity of 0.22-0.28 mu m/s for kinesincoated microspheres and 0.34-0.39 mu m/s for dynein-coated microspheres. This bidirectional nanotransport goes beyond conventional unidirectional transport to achieve more complex artificial nanotransport in vitro. Biotechnol. Bioeng. 2008;101: 1-8. (C) 2008 Wiley Periodicals, Inc.
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