期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 101, 期 1, 页码 190-195出版社
WILEY-BLACKWELL
DOI: 10.1002/bit.21877
关键词
fast loading; cell array; long-term monitor; zero flow
资金
- Department of Science and Technology in China, Chinese Natural Science Foundation
We present a simple technique for cell loading, culturing, and phenotypic study in a multi-chamber microfluidic device made of polydimethylsiloxane (PDMS). This technique is based on the use of degassing induced aspiration of PDMS which allows loading cells into microcavities within 1 min. A large number of triangle cavities art, patterned aside main flow channels with narrow connections so that cells can be loaded by aspirating into each cavity. In our device, high throughput and long-term monitoring can be done with minimum shear force of the flow. As a demonstration, we show a controlled loading at single cell level and the phenotypic variation of gene expression of the yeast strain w303 as a function of copper ion concentration of the medium. Biotechnol. Bioeng. 2008;101: 190-195. (C) 2008 Wiley Periodicals, Inc.
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