期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1402, 期 -, 页码 82-93出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2015.05.016
关键词
Polymer monolith; ISEC; Incomplete polymerization; HILIC
资金
- project Enhancement of R&D Pools of Excellence at the University of Pardubice [CZ.1.07/2.3.00/30.0021]
- Operational Program Education for Competitiveness - European Social Fund Ministry of Education, Youth and Sports of the Czech Republic [CZ.1.07./2.3.00/20.0254]
Zwitterionic methacrylate based polymeric monolithic columns were prepared in two-step polymerizations, with reduced polymerization times. Characteristic properties such as hydrodynamic permeability, porosity, retention factors, and pore size distribution charts were used for column evaluation. A scaffold column was fabricated by polymerization of poly(lauryl methacrylate-co-tetraethyleneglycol dimethacrylate) and was used without further modification as a support for a poly(N,N-dimethyl-N-methacryloxyethyl-N-(3-sulfopropyl)ammonium betaine-co-bisphenol A glycerolate dimethacrylate) second monolith layer with zwitterionic functionality, for HILIC separations. An additional internal structure was formed by the second monolithic layer. The fabrication procedure was reproducible with RSD < 5%, Field emission scanning electron microscopy has also been used to investigate column pore morphology, using a novel technique where the polymeric material is imaged directly, without coverage with a conducting film or particles. The new polar monolithic columns were used for HILIC separations of phenolic acids, flavones, nucleosides, and bases of nucleic acids, with similar efficiencies but different selectivities for zwitterionic methacrylate monolithic columns recently prepared by single step polymerization. (C) 2015 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据