期刊
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
卷 59, 期 3, 页码 163-169出版社
WILEY
DOI: 10.1002/bab.1001
关键词
cartilage defect; nonviral gene vector; reverse transfection; three dimensional; mesenchymal stem cells
资金
- National Natural Science Foundation of People's Republic of China [30873173, 81001410]
- Fundamental Research Funds for Central University [81001410]
- Zhejiang Provincial Natural Science Foundation of People's Republic of China [R2090176]
- People's Republic of China-Japan Scientific Cooperation Program [81011140077]
- NSFC (People's Republic of China)
- JSPS (Japan)
- Zijin Program of Zhejiang University [188020-544802]
This study evaluated the potential of utilizing transfected pTGF beta-1 gene-engineered rat mesenchymal stem cells (MSCs) using nonviral vector to promote cartilage regeneration. Pullulanspermine was used as the nonviral gene vector and gelatin sponge was used as the scaffold. MSCs were engineered with TGF-beta 1 gene with either the three-dimensional (3D) reverse transfection system or the two-dimensional (2D) conventional transfection system. For the 3D reverse transfection system, pullulanspermine/pTGF-beta 1 gene complexes were immobilized to the gelatin sponge, followed by the seeding of MSCs. Pullulanspermine/pTGF-beta 1 gene complexes were delivered to MSCs cultured in the plate to perform the 2D conventional transfection system, and then MSCs were seeded to the gelatin sponge. Then, TGF-beta 1 gene-transfected MSC seeded gelatin sponge was implanted to the full-thickness cartilage defect. Compared with the control group, both groups of TGF-beta 1 gene-engineered MSCs improved cartilage regeneration through optical observation and histology staining. So, with pullulanspermine as the nonviral vector, TGF-beta 1-gene engineered MSCs can induce cartilage regeneration in vivo.
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