期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1409, 期 -, 页码 1-10出版社
ELSEVIER
DOI: 10.1016/j.chroma.2015.07.025
关键词
Molecularly imprinted polymer; Molecular recognition; DFT calculations; Solid phase extraction; Olive oil; Deltamethrin
资金
- FEDER
- Programa Operacional Regional do Alentejo (InAlentejo) Operation [ALENT-07-0262-FEDER-001871]
- FEDER Funds through the Operational Programme for Competitiveness Factors -COMPETE
- National Funds through FCT - Foundation for Science and Technology [PEst-OE/AGR/UI0115/2014, PEst-OE/QUI/UI0619/2014]
- Fundacao para a Ciencia e a Tecnologia (FCT) [SFRH/BPD/72182/2010]
- INMOLFARM - Molecular Innovation and Drug Discovery [ALENT-57-2011-20]
- FEDER-INALENTEJO program [ALENT-07-0224-FEDER-001743]
- [PTDC/AGR-ALI/117544/2010]
- Fundação para a Ciência e a Tecnologia [PTDC/AGR-ALI/117544/2010] Funding Source: FCT
This work firstly addresses the design and development of molecularly imprinted systems selective for deltamethrin aiming to provide a suitable sorbent for solid phase (SPE) extraction that will be further used for the implementation of an analytical methodology for the trace analysis of the target pesticide in spiked olive oil samples. To achieve this goal, a preliminary evaluation of the molecular recognition and selectivity of the molecularly imprinted polymers has been performed. In order to investigate the complexity of the mechanistic basis for template selective recognition in these polymeric matrices, the use of a quantum chemical approach has been attempted providing new insights about the mechanisms underlying template recognition, and in particular the crucial role of the crosslinker agent and the solvent used. Thus, OFT calculations corroborate the results obtained by experimental molecular recognition assays enabling one to select the most suitable imprinting system for MISPE extraction technique which encompasses acrylamide as functional monomer and ethylene glycol dimethacrylate as crosslinker. Furthermore, an analytical methodology comprising a sample preparation step based on solid phase extraction has been implemented using this tailor made imprinting system as sorbent, for the selective isolation/pre-concentration of deltamethrin from olive oil samples. Molecularly imprinted solid phase extraction (MISPE) methodology was successfully applied for the clean-up of spiked olive oil samples, with recovery rates up to 94%. (C) 2015 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据