期刊
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
卷 59, 期 5, 页码 388-394出版社
WILEY
DOI: 10.1002/bab.1031
关键词
chitosan; nerve growth factor; genipin; nerve conduits
资金
- Hi-Tech Research and Development Program of People's Republic of China (863 Program) [2012AA020502]
- Nature Science Foundation of People's Republic of China [30970713, 81171457]
- Basic Research Program of Jiangsu Education Department [BK2009158]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
We have developed a nerve conduit made up of chitosan, on which nerve growth factor (NGF) was immobilized via genipin cross-linking. The nerve conduit was used to bridge a 10-mm-long sciatic nerve gap in rats. At 24 weeks after surgery, electrophysiological assessment, behavioral analysis, and histological examination were conducted to evaluate the outcomes of peripheral nerve repair. The nerve conduit allowed nerve reconstruction between two stumps and reinnervation of the target gastrocnemius muscle. For two groups of rats repaired respectively by the nerve conduit and autologous nerve graft, the density of regenerated axons was 3.55 +/- 0.51 and 3.91 +/- 0.14 (P = 0.712), and the cross-sectional area of target muscles was 1,159.68 +/- 305.85 and 1,307.06 +/- 301.25 (P = 0.922), respectively, without significant differences between the two groups. Our data suggest the feasibility of using chitosan-based, NGF-loaded nerve conduits for peripheral nerve repair.
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