期刊
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
卷 58, 期 5, 页码 335-344出版社
WILEY
DOI: 10.1002/bab.44
关键词
elastomer; porogen; microelectromechanical systems; scaffold; vascular graft
资金
- American Heart Association
- National Institute of Biomedical Imaging and Bioengineering [R21EB009795]
- Cancer Prevention and Research Institute of Texas [RP110412]
- National Science Foundation [0954109]
- Division Of Materials Research
- Direct For Mathematical & Physical Scien [1313553, 0954109] Funding Source: National Science Foundation
Porogen leaching is a widely used and simple technique for the creation of porous scaffolds in tissue engineering. Sodium chloride (NaCl) is the most commonly used porogen, but the current grinding and sieving methods generate salt particles with huge size variations and cannot generate porogens in the submicron size range. We have developed a facile method based on the principles of crystallization to precisely control salt crystal sizes down to a few microns within a narrow size distribution. The resulting NaCl crystal size could be controlled through the solution concentration, crystallization temperature, and crystallization time. A reduction in solution temperature, longer crystallization times, and an increase in salt concentration resulted in an increase in NaCl crystal sizes due to the lowered solubility of the salt solution. The nucleation and crystallization technique provides superior control over the resulting NaCl size distribution (13.78 +/- 1.18 mu m), whereas the traditional grinding and sieving methods produced NaCl porogens 13.89 +/- 12.49 mu m in size. The resulting NaCl porogens were used to fabricate scaffolds with increased interconnectivity, porous microchanneled scaffolds, and multiphasic vascular grafts. This new generation of salt porogen provides great freedom in designing versatile scaffolds for various tissue-engineering applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据