期刊
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
卷 58, 期 5, 页码 345-352出版社
WILEY-BLACKWELL
DOI: 10.1002/bab.45
关键词
fibrin; gelatin; cartilage tissue engineering; stem cells; growth factor delivery; hydrogel
资金
- European Research Council [258463]
- European Research Council (ERC) [258463] Funding Source: European Research Council (ERC)
Articular cartilage has a limited capacity for self-renewal and repair. Tissue engineering of cartilage in vitro has been proposed as a solution to this problem; however, this approach is costly and requires a significant amount of time to grow the graft. An alternative approach is to implant chondroprogenitor cells seeded within a growth factor delivery scaffold directly into the defect site to promote tissue regeneration. The objective of this study was to develop a biocompatible growth factor delivery system capable of promoting chondrogenesis of infrapatellar fat pad (IFP)-derived stem cells. Transforming growth factor beta-1 (TGF-beta 1) was loaded into gelatin microspheres and incorporated into fibrin hydrogels containing IFP-derived stem cells. The release of TGF-beta 1 was quantified using an enzyme-linked immunosorbent assay, whereas chondrogenesis was demonstrated histologically and by quantifying sulfated glycosaminoglycan production after 21 days of in vitro culture. TGF-beta 1 loaded into gelatin microspheres appeared to be as effective in promoting chondrogenesis of IFP-derived stem cells as adding TGF-beta 1 directly to the medium. The influence of different microsphere fabrication parameters and TGF-beta 1 loading concentrations was also investigated but appeared to only have a small effect on subsequent chondrogenesis. The development of such growth factor delivery systems in combination with IFP-derived stem cells represents a potential new strategy for cartilage defect repair.
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