期刊
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
卷 58, 期 4, 页码 271-276出版社
WILEY
DOI: 10.1002/bab.39
关键词
chondrogenesis; human mesenchymal stem cells; hyaluronic acid; transforming growth factor-beta3
资金
- National Research Foundation of Korea [2009-0086518, 2009-0092213]
- Ministry of Health and Welfare, Republic of Korea [A101539]
- Korea Health Promotion Institute [A101539] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2009-0092213, 2009-0086518] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
This study was designed to evaluate the additive effects of transforming growth factor-beta3 (TGF-beta 3) and hyaluronic acid (HA) on chondrogenic differentiation of human mesenchymal stem cells (hMSCs). The hMSCs were cultured on collagen type I-, HA-, or fibronectin-coated cell culture dishes with or without TGF-beta 3 added to the culture medium. Four weeks after cell culture, chondrogenic differentiation of hMSCs was determined by evaluating the expression of cartilage-specific markers using real-time polymerase chain reaction, immunocytochemistry, and Western blot analysis. hMSCs cultured on HA-coated dishes with TGF-beta 3 supplementation revealed a prominent increase in collagen type II, aggrecan, and Sox9. When hMSCs were cultured without TGF-beta 3 supplementation, only hMSCs cultured on HA-coated dishes showed prominent expression of the cartilage-specific markers. This study shows that chondrogenic differentiation of hMSCs can be enhanced additively by interactions with both a specific cell-adhesion matrix and a soluble growth factor.
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