期刊
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
卷 55, 期 -, 页码 45-52出版社
PORTLAND PRESS LTD
DOI: 10.1042/BA20090277
关键词
antimicrobial activity; biosurfactant; high-throughput bioprocessing; microbioreactor (mu BR); microfluidic bioreactor; rhamnolipid
资金
- Teesside University [04/02/10]
- Enterprise Development Fund
- FROPTOP
- UK Bioscience for Business Knowledge Transfer Network
A low-cost mu BR (microbioreactor) made from PTFE [poly(tetrafluoroethylene)] was used to cultivate a model organism, Pseudomonas aeruginosa DS10-129. The progress of bioprocessing was monitored by comparing the growth of the organism in a mu BR, a conventional bench scale bioreactor and a shake flask. Under the mu BR conditions, the organism produced 23 mg/ml of pyocyanin that had antimicrobial effects against Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas teessidea and Pseudomonas clemancea. Furthermore, it produced a total of 106 mu g/ml of effective biosurfactants consisting of dirhamnolipids (RL2) and monorhamnolipids (RL1). The biosurfactants reduced the surface tension of distilled water from 72 to 27.9 mN/m and emulsified kerosene by 71.30%. The pyocyanin and rhamnolipids were produced during the exponential and stationary phases of growth respectively. The results of the mu BR were comparable to those obtained using conventional scale methods.
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