期刊
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
卷 55, 期 -, 页码 169-174出版社
WILEY
DOI: 10.1042/BA20090314
关键词
fermentor pressure; glucose feedback; high-cell-density cultivation; human-like collagen; oxygen-enriched air; recombinant Escherichia coli
资金
- National High Technology Research and Development Program of China [2006AA02Z246, 2007AA03Z456]
- National Natural Science Foundation of China [20776119]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20096101120023]
- Scientific Research Program of Shaanxi Provincial Department of Education, China [07JK417]
- Shaanxi Key Subject Program, China
The effects of different methods for elevating the OTR (oxygen transfer rate) during foreign gene expression and the cell growth of recombinant Escherichia coli BL2I were investigated. Two strategies were introduced to control DO (dissolved oxygen) levels in the fermentation broth: (i) increasing fermentor pressure and (ii) supplying oxygen-enriched air. These two methods were compared with the glucose feedback model, which acted as the control. By adopting a fed-batch method of cultivation, the cell yield coefficient (Y-x/s), accumulation of acetic acid and volumetric product yield (Y-p) were measured or estimated. Adoption of these two methods led to an improvement in the OTR. The cell density and volumetric product yield in the cultivation controlled by increasing the fermentor pressure reached 77 g . l(-1) (dry cell weight) and 14 g . l(-1) respectively, which were much higher than those obtained with the strategy of supplying oxygen-enriched air (48 and 6 g . l(-1) respectively) and in the control (46 and 7 g . l(-1) respectively). The results indicate that increasing fermentor pressure is an effective way to enhance the OTR and recombinant protein (human-like collagen) productivity.
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