期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1416, 期 -, 页码 64-73出版社
ELSEVIER
DOI: 10.1016/j.chroma.2015.08.071
关键词
Organic-silica hybrid monolithic capillary column; Pipette tip solid phase extraction; Capillary liquid chromatography-mass spectrometry; Derivatization; Gibberellin
资金
- National Natural Science Foundation of China [91217309, 21475098]
- Natural Science Foundation of Hubei Province, China [2014CFA002]
A newly improved one-pot method, based on thiol-ene click chemistry and sol-gel approach in microemulsion system, was developed for the preparation of C-8/PO(OH)(2)-silica hybrid monolithic capillary column. The prepared monolith possesses large specific surface area, narrow mesopore size distribution and high column efficiency. The monolithic column was demonstrated to have cation exchange/reversed-phase (CX/RP) mixed-mode retention for analytes on nano-liquid chromatography (nano-LC). On the basis of the developed nano-LC system with MS detector coupled to pipette tip solid phase extraction (PT-SPE) and derivatization process, we then realized simultaneous determination of 10 gibberellins (GM) with low limits of detection (LODs, 0.003-0.025 ng/mL). Furthermore, 6 endogenous GAs in only 5 mg rice leaves (fresh weight) were successfully detected and quantified. The developed PT-SPE-nano-LC-MS strategy may offer promising applications in the determination of low abundant bioactive molecules from complex matrix. (C) 2015 Elsevier B.V. All rights reserved.
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