期刊
BIOTECHNOLOGY ADVANCES
卷 32, 期 2, 页码 415-428出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2013.12.004
关键词
Cyclodextrin glycosyltransferase (CGTase); Heterologous expression; Molecular modification; Directed evolution
资金
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- 111 Project [111-2-06]
- Fundamental Research Funds for the Central Universities [JUSRP211A29]
- 863 Program [2011AA100905]
- 973 Program [2012CB720806]
Cyclodextrin glycosyltransferase (CGTase) is an important enzyme with multiple functions, in particular the production of cyclodextrins. It is also widely applied in baking and carbohydrate glycosylation because it participates in various types of catalytic reactions. New applications are being found with novel CGTases being isolated from various organisms. Heterologous expression is performed for the overproduction of CGTases to meet the requirements of these applications. In addition, various directed evolution techniques have been applied to modify the molecular structure of CGTase for improved performance in industrial applications. In recent years, substantial progress has been made in the heterologous expression and molecular engineering of CGTases. In this review, we systematically summarize the heterologous expression strategies used for enhancing the production of CGTases. We also outline and discuss the molecular engineering approaches used to improve the production, secretion, and properties (e.g., product and substrate specificity, catalytic efficiency, and thermal stability) of CGTase. (C) 2013 Elsevier Inc. All rights reserved.
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