期刊
BIOTECHNOLOGY ADVANCES
卷 32, 期 2, 页码 280-289出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2013.10.009
关键词
Protista; Metamonada; Giardia; Giardia duodenalis; Giardiasis; Diagnosis; Detection; Genetic characterisation
资金
- Australian Research Council (ARC)
- National Health and Medical Research Council (NHMRC)
- Melbourne Water Corporation
- Alexander von Humboldt Foundation
- Freie University Berlin
Giardiasis is a gastrointestinal disease of humans and other animals caused by species of parasitic protists of the genus Giardia. This disease is transmitted mainly via the faecal oral route (e.g., in water or food) and is of socioeconomic importance worldwide. The accurate detection and genetic characterisation of the different species and population variants (usually referred to as assemblages and/or sub-assemblages) of Giardia are central to understanding their transmission patterns and host spectra. The present article provides a background on Giardia and giardiasis, and reviews some key techniques employed for the identification and genetic characterisation of Giardia in biological samples, the diagnosis of infection and the analysis of genetic variation within and among species of Giardia. Advances in molecular techniques provide a solid basis for investigating the systematics, population genetics, ecology and epidemiology of Giardia species and genotypes as well as the prevention and control of giardiasis. (C) 2013 Elsevier Inc. All rights reserved.
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