期刊
BIOTECHNOLOGY ADVANCES
卷 31, 期 6, 页码 804-810出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2013.04.003
关键词
Genome engineering; lambda Red recombination; Multiplex automated genome engineering
资金
- Next-Generation BioGreen 21 Program [PJ009034012013]
- Intelligent Synthetic Biology Center of the Global Frontier Project
- Ministry of Science, ICT & Future Planning, Korea [2011-0031956]
- National Junior Research Fellowship from the National Research Foundation of Korea [2011-0012414]
- National Research Foundation of Korea [2011-0012414] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Rural Development Administration (RDA), Republic of Korea [PJ009034012013] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Genome engineering has been developed to create useful strains for biological studies and industrial uses. However, a continuous challenge remained in the field: technical limitations in high-throughput screening and precise manipulation of strains. Today, technical improvements have made genome engineering more rapid and efficient. This review introduces recent advances in genome engineering technologies applied to Escherichia coli as well as multiplex automated genome engineering (MAGE), a recent technique proposed as a powerful toolkit due to its straightforward process, rapid experimental procedures, and highly efficient properties. (C) 2013 Elsevier Inc. All rights reserved.
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