期刊
BIOTECHNOLOGY ADVANCES
卷 31, 期 8, 页码 1319-1332出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2013.05.006
关键词
beta-lactam antibiotics; Escherichia coli; Industrial enzyme; Penicillin; Penicillin chemistry; Penicillin G acylase; Posttranslational processing; Recombinant protein; Semisynthetic antibiotics
资金
- Natural Sciences and Engineering Research Council (NSERC)
- Canada Research Chair (CRC) program of Canada
In light of unrestricted use of first-generation penicillins, these antibiotics are now superseded by their semisynthetic counterparts for augmented antibiosis. Traditional penicillin chemistry involves the use of hazardous chemicals and harsh reaction conditions for the production of semisynthetic derivatives and, therefore, is being displaced by the biosynthetic platform using enzymatic transformations. Penicillin G acylase (PGA) is one of the most relevant and widely used biocatalysts for the industrial production of beta-lactam semisynthetic antibiotics. Accordingly, considerable genetic and biochemical engineering strategies have been devoted towards PGA applications. This article provides a state-of-the-art review in recent biotechnological advances associated with PGA, particularly in the production technologies with an emphasis on using the Escherichia coli expression platform. (C) 2013 Elsevier Inc. All rights reserved.
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