期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1379, 期 -, 页码 65-73出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2014.12.053
关键词
Parabens; Methacrylate monolithic columns; Capillary-LC-MS; Human serum; Human urine; DLLME
资金
- MINECO of Spain [CTQ2014-52765-R]
- FEDER funds [CTQ2014-52765-R]
- Regional Government of Andalusia [P09-CTS-4470]
- MINECO
- University of Granada
A method for the determination of parabens in human urine and serum by capillary liquid chromatography (cLC) with UV-Vis and mass spectrometry (MS) detection using methacrylate ester-based monolithic columns has been developed. The influence of composition of polymerization mixture was studied. The optimum monolith was obtained with butyl methacrylate monomer at 60/40% (wt/wt) butyl methacrylate/ethylene dimethacrylate ratio and 50 wt% porogens (composed of 36 wt% of 1,4-butanediol, 54wt% 1-propanol and 10wt% water). Baseline resolution of analytes was achieved through a mobile phase of acetonitrile/water in gradient elution mode. Additionally, dispersive liquid-liquid microextraction (DLLME) was combined with both cLC-UV-Vis and cLC-MS to achieve the determination of parabens in human urine and serum samples with very low limits of detection. Satisfactory intra- and inter-day repeatabilities were obtained in UV-Vis and MS detection, although the latter provided lower detection limits (up to 300-fold) than the UV-Vis detection. Recoveries for the target analytes from spiked biological samples ranged from 95.2% to 106.7%. The proposed methodology for the ultra-low determination of parabens in human urine and serum samples is simple and fast, the consumption of reagents is very low, and very small samples can be analyzed. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据