期刊
BIOTECHNIQUES
卷 54, 期 6, 页码 321-+出版社
BIOTECHNIQUES OFFICE
DOI: 10.2144/000114039
关键词
comparative biology; cross-species capture; DNA hybridization capture; next-generation sequencing; protein-coding genes; phylogenetics; target enrichment
资金
- National Science Foundation (DEB) [1132229]
- Leading Academic Discipline Project of the Shanghai Municipal Education Commission [S30701]
- Division Of Environmental Biology
- Direct For Biological Sciences [1132229] Funding Source: National Science Foundation
DNA hybridization capture combined with next generation sequencing can be used to determine the sequences of hundreds of target genes across hundreds of individuals in a single experiment. However, the approach has thus far only been successfully applied to capture targets that are highly similar in sequence to the bait molecules. Here we introduce modifications that extend the reach of the method to allow efficient capture of highly divergent homologous target sequences using a single set of baits. These modifications have important implications for comparative biology.
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