期刊
BIOTECHNIQUES
卷 52, 期 6, 页码 381-385出版社
BIOTECHNIQUES OFFICE
DOI: 10.2144/0000113878
关键词
FACS; parvalbumin; pyramidal; nanoCAGE; RNA seq
资金
- JSPS
- Japanese Ministry of Education, Culture, Sports, Science & Technology (MEXT) [17021047]
- Human Frontiers Science Program grant [HFSP PGP 0018/2007-C]
- 7th Framework grant (Dopaminet)
- MEXT [20241047]
- National Human Genome Research Institute [U54 HG004557]
- MEXT
- Omics Science Center
- Grants-in-Aid for Scientific Research [17021047] Funding Source: KAKEN
Efficient isolation of specific, intact, living neurons from the adult brain is problematic due to the complex nature of the extracellular matrix consolidating the neuronal network. Here, we present significant improvements to the protocol for isolation of pure populations of neurons from mature postnatal mouse brain using fluorescence activated cell sorting (FAGS). The 10-fold increase in cell yield enables cell-specific transcriptome analysis by protocols such as GAGE and and RNA seq.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据