期刊
BIOTECHNIQUES
卷 50, 期 1, 页码 32-40出版社
FUTURE SCI LTD
DOI: 10.2144/000113569
关键词
SPR; SPR imaging; proteomics; label-free; bioengineering; antibody array; protein array; antibody
资金
- National Science Foundation [0912245]
Surface plasmon resonance (SPR) is a label-free detection method by which molecular interactions may be analyzed on a surface. Binding data are collected in real time, allowing the determination of interaction kinetics. SPR imaging (SPRi), the focus of this review, improves upon the efficiency of SPR by facilitating analysis of multiple interactions simultaneously. Here we summarize the principles of SPRi, provide examples of how SPRi arrays can be fabricated, and illustrate the utility of SPRi through example applications from the fields of proteomics, genomics and bioengineering.
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