期刊
BIOTECHNIQUES
卷 51, 期 1, 页码 29-+出版社
BIOTECHNIQUES OFFICE
DOI: 10.2144/000113701
关键词
magnetic tweezers; confocal microscopy; hydrogel; traction force; cytoskeleton; structure-mechanics relationships
资金
- Wellcome Fund Career Award at the Scientific Interface
- National Science Foundation/National Nanotechnology Infrastructure Network (NSF/NNIN) at University of California-Santa Barbara [ECS-0335765]
- Santa Barbara Cottage Hospital
- National Institutes of Health/National Center for Research Resources (NIH/NCRR) [1S10RR017753-01]
We have designed and built a magnetic tweezers device that enables the application of calibrated stresses to soft materials while simultaneously measuring their microscale deformation using confocal microscopy. Unlike previous magnetic tweezers designs, our device is entirely portable, allowing easy use on microscopes in core imaging facilities or in collaborators' laboratories. The imaging capabilities of the microscope are unimpaired, enabling the 3-D structures of fluorescently labeled materials to be precisely determined under applied load. With this device, we can apply a large range of forces (similar to 1-1200 pN) over micron-scale contact areas to beads that are either embedded within 3-D matrices or attached to the surface of thin slab gels. To demonstrate the usefulness of this instrument, we have studied two important and biologically relevant materials: polyacrylamide-based hydrogel films typical of those used in cell traction force microscopy, and reconstituted networks of microtubules, essential cytoskeletal filaments.
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