期刊
BIOTECHNIQUES
卷 48, 期 1, 页码 47-52出版社
BIOTECHNIQUES OFFICE
DOI: 10.2144/000113317
关键词
hepatocytes; co-culture; layered cell patterning; cellular interactions; fibroblasts
资金
- National Institutes of Health (NIH) [R01 DK43371, K08 DK066040, K18 DK076819]
- NIH BioMEMS Resource Center [P41 EB02503]
- Shriners Hospitals for Children
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [P41EB002503] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [P30DK040561, K08DK066040, K18DK076819, R01DK043371] Funding Source: NIH RePORTER
Microfabrication and micropatterning techniques in tissue engineering offer great potential for creating and controlling microenvironments in which cell behavior can be observed. Here we present a novel approach to generate layered patterning of hepatocytes on micropatterned fibroblast feeder layers using microfabricated polydimethylsiloxane (PDMS) stencils. We fabricated PDMS stencils to pattern circular holes with diameters of 500 mu m. Hepatocytes were co-cultured with 3T3-J2 fibroblasts in two types of patterns to evaluate and characterize the cellular interactions in the co-culture systems. Results of this study demonstrated uniform intracellular albumin staining and E-cadherin expression, increased liver-specific functions, and active glycogen synthesis in the hepatocytes when the heterotypic interface between hepatocytes and fibroblasts was increased by the layered patterning technique. This patterning technique can be a useful experimental tool for applications in basic science, drug screening, and tissue engineering, as well as in the design of bioartificial liver devices.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据