期刊
BIOTECHNIC & HISTOCHEMISTRY
卷 87, 期 7, 页码 433-438出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/10520295.2012.696700
关键词
C-banding; euchromatin; fluorescence; fluorochromes; Giemsa; heterochromatin
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [proc. 09/15780-6]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [proc. 083/2007]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
The detection of regions of heterochromatin has been the subject of intense investigation. We investigated an adaptation of the commonly used technique by replacing the nonfluorescent dye, Giemsa, by a fluorescent one, propidium iodide. This adaptation produces greater contrast of the heterochromatic bands in metaphase chromosomes and can be especially valuable when the organisms studied possess heterochromatin that is pale and difficult to visualize. We discuss the interactions of these two dyes with DNA and the excitation of the fluorescent dye when irradiated with ultraviolet light.
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