期刊
ENEURO
卷 5, 期 2, 页码 -出版社
SOC NEUROSCIENCE
DOI: 10.1523/ENEURO.0276-17.2018
关键词
axon growth; axon injury; nerve regeneration; translational regulation
资金
- Dr. Miriam and Sheldon G. Adelson Medical Research Foundation
- European Research Council (Neurogrowth)
- Israel Science Foundation [1284/13]
- Minerva Foundation
Transcriptional events leading to outgrowth of neuronal axons have been intensively studied, but the role of translational regulation in this process is not well understood. Here, we use translatome analyses by ribosome pull-down and protein synthesis characterization by metabolic isotopic labeling to study nerve injury and axon outgrowth proteomes in rodent dorsal root ganglia (DRGs) and sensory neurons. We identify over 1600 gene products that are primarily translationally regulated in DRG neurons after nerve injury, many of which contain a 5' UTR cytosine-enriched regulator of translation (CERT) motif, implicating the translation initiation factor Eif4e in the injury response. We further identified approximately 200 proteins that undergo robust de novo synthesis in the initial stages of axon growth. ApoE is one of the highly synthesized proteins in neurons, and its receptor binding inhibition or knockout affects axon outgrowth. These findings provide a resource for future analyses of the role of translational regulation in neuronal injury responses and axon extension.
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