期刊
BIOSYSTEMS
卷 97, 期 3, 页码 160-164出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biosystems.2009.06.002
关键词
Dual-fluorescence system; Lysine biosynthesis; Promoter activity
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan
Stochastic dynamics of promoter activity in bacterial cells were studied by using a dual-fluorescence reporter system of protein expression. The dual-fluorescence reporter system enabled us to calculate the amplitude of intrinsic noise generated during transcription and translation. By fitting the experimental data to a simple stochastic model of protein expression, we could estimate parameters representing the stochastic transition between the active and inactive states of a promoter. Using the system, we analyzed the stochastic dynamics of promoter activation of genes in the lysine biosynthesis pathway in Escherichia coli. We found that the promoter of lysA has a significantly slower transition rate between active and inactive states than other promoters in the lysine biosynthesis pathway. The infrequent switching between active and inactive states can be a dominant source of noise in lysA expression. Analysis using the dual-fluorescence reporter system provided a better understanding of stochastic dynamics in promoter activation. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
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