期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 115, 期 3, 页码 729-738出版社
WILEY
DOI: 10.1002/bit.26508
关键词
acetate; glyoxylate shunt; itaconic acid; metabolic engineering; synthetic biology
资金
- Global Research Laboratory Program [NRF-2016K1A1A2912829]
- Ministry of Science, ICT and Future Planning (Korea) [NRF-2016M3D3A1A 019132 37]
- Korea Institute of Energy Technology Evaluation and Planning (KETEP)
- Ministry of Trade Industry & Energy (MOTIE) of the Republic of Korea [20174 030201600]
Utilization of abundant and cheap carbon sources can effectively reduce the production cost and enhance the economic feasibility. Acetate is a promising carbon source to achieve cost-effective microbial processes. In this study, we engineered an Escherichia coli strain to produce itaconic acid from acetate. As acetate is known to inhibit cell growth, we initially screened for a strain with a high tolerance to 10 g/L of acetate in the medium, and the W strain was selected as the host. Subsequently, the WC strain was obtained by overexpression of cad (encoding cis-aconitate decarboxylase) using a synthetic promoter and 5 UTR. However, the WC strain produced only 0.13 g/L itaconic acid because of low acetate uptake. To improve the production, the acetate assimilating pathway and glyoxylate shunt pathway were amplified by overexpression of pathway genes as well as its deregulation. The resulting strain, WCIAG4 produced 3.57 g/L itaconic acid (16.1% of theoretical maximum yield) after 88 hr of fermentation with rapid acetate assimilation. These efforts support that acetate can be a potential feedstock for biochemical production with engineered E. coli.
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