Construction and application of amperometric sarcosine biosensor based on SOxNPs/AuE for determination of prostate cancer

An improved amperometric sarcosine biosensor was constructed based on covalent immobilization of sarcosine oxidase nanoparticles (SOxNPs) onto gold electrode (AuE). The SOxNPs/AuE was characterized by scanning electron microscopy (SEM), fourier transform infrared (FTIR) spectroscopy and electrochemical impedance spectroscopy (EIS) at different stages of its construction. The biosensor worked optimally within 2 s at a potential of 1.0 V, against Ag/AgCl, pH 6.5 and 35 degrees C. A linear relationship was observed between sarcosine concentration range, 0.1-100 mu M and the biosensor response i.e. current in mA under optimum conditions. The biosensor offered a low detection limit of 0.01 mu M and gratifying storage stability. The SOxNPs/AuE was unaffected by a number of serum substances at their physiological concentrations. The biosensor measured sarcosine level in sera collected from persons suffering from prostate cancer (mean13.5 mu M, n = 8), which was significantly higher (p < 0.01) than those in apparently healthy persons (mean 2.2 mu M, n = 8). The SOxNPs/Au electrode was reused 300-times during the span of 180 days, with only 10% loss in its initial activity while being stored dry at 4 degrees C.