期刊
BIOSENSORS & BIOELECTRONICS
卷 121, 期 -, 页码 90-95出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.08.071
关键词
Homogeneous photoelectrochemical assay; Tetracationic porphyrin; Label-free; Methyltransferase activity analysis
类别
资金
- National Natural Science Foundation of China [31501570, 21575074, 21775083]
- Research Foundation for Distinguished Scholars of Qingdao Agricultural University [663-1115003]
- Major Basic Research Program of Natural Science Foundation of Shandong Province [ZR2018ZC0127]
- Special Foundation for Distinguished Taishan Scholar of Shandong Province [ts201511052]
This work reports the first demonstration of an oligonucleotide-modulated label-free homogeneous photoelectrochemical (PEC) biosensing platform based on the adsorption of tetracationic porphyrin (denoted as TMPyP here) onto 1-naphthalenesulfonate anion (NS-)-grafted indium tin oxide electrode (denoted as TMPyP-NS--ITO), which generates a stable and rapid photocurrent response. We found that when NS--ITO electrode was subjected to single-stranded oligonucleotide (ssON) before TMPyP adsorption, a remarkable enhancement of photocurrent intensity was observed from the resulted TMPyP-ssON-NS--ITO electrode with high specificity towards oligonucleotide. A series of investigations were carried out to understand the mechanism of this oligonucleotide-modulated photocurrent enhancement phenomenon. Moreover, the studies of this robust photo current enhancement mechanism was successfully extended to develop a signal-on homogeneous PEC biosensing platform for, as a proof-of-concept, label-free M.SssI methyltransferase activity analysis through a judiciously and compatibly engineered signal transduction strategy consisted of hairpin-shaped oligonucleotide probe, restriction endonuclease HpaII, and Exonuclease L The rationally designed homogeneous PEC biosensor exhibit sensitive PEC response toward M.SssI methyltransferase with a low detection limit of 3.5 mU/mL and a wide linear range from 0.01 to 120 U/mL. Additionally, we show that our homogeneous PEC biosensing platform can be also utilized to screen methyltransferase inhibitors. Therefore, this work will provide a distinctive paradigm for versatile homogeneous PEC biosensing platform that can be used as potential powerful tool toward innovative label-free bioanalytical purposes.
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