期刊
BIOSENSORS & BIOELECTRONICS
卷 121, 期 -, 页码 72-79出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.08.044
关键词
Self-assembled monolayer; Immobilization of biomolecules; Addressable immobilization; Michael addition; Cyclic voltammetry; Surface plasmon resonance
类别
资金
- Tunisian Ministry of Higher Education and Scientific Research
- Tunisia-Germany bilateral project RESPONSE - Tunisian Ministry of Higher Education and Scientific Research
- German Federal Ministry of Education and Research (BMBF)
Electrically addressed covalent immobilization of biomolecules to the defined electrodes of an electrode array is described. It is based on Michael addition of the thiol group of biomolecules to alpha,beta-unsaturated carbonyl groups of benzoquinone. This click reaction was tested by immobilization of a number of thiolated compounds on the simplest array consisting of two gold electrodes coated by a self-assembled monolayer of benzoquinone-terminated hexanethiol. Electrically controlled binding of hexanethiol, ferrocenylhexanethiol, human serum albumin and thiol-terminated single-stranded DNA (ssDNA) was investigated. The binding was studied using cyclic voltammetry, X-ray photoelectron spectroscopy and surface plasmon resonance. The reaction requires the oxidized state of the benzoquinone moiety; this can be reached by applying of a moderate anodic potential to the electrode. Surface plasmon resonance measurements demonstrated that the thiol-modified ssDNA immobilized by this technique binds complementary synthetic oligonucleotides or PCR-amplified DNA fragments. The developed technology of electrical addressing of covalent immobilization can be applied for fabrication of sensor arrays.
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