期刊
BIOSENSORS & BIOELECTRONICS
卷 123, 期 -, 页码 223-229出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.08.064
关键词
Human norovirus; Specific binding peptides; Biosensor; Electrochemical impedance spectroscopy
类别
资金
- Ministry of Oceans and Fisheries [20160122]
- Cooperative Research Program for Agriculture Science and Technology Development Rural Development Administration, Republic of Korea [PJ01280901]
- Ministry of Trade, Industry & Energy (MI, Korea) [20000773]
Human noroviruses cause acute foodbome gastroenteritis outbreaks worldwide. In this study, a highly sensitive and selective electrochemical biosensor was fabricated for the detection of human norovirus using novel peptides as recognition elements. The electrochemical biosensor was fabricated by assembling of eight novel peptides separately on the gold electrode and investigated their efficiencies for sensing human noroviruses. Among eight peptides, NoroBP peptide coated onto the gold electrode exhibited a high binding affinity towards human noroviruses, resulting a progressive decrease in current signals with increasing concentration of human norovirus (0-10(5) copies/mL). As a result, NoroBP-nonFoul(FlexL)(2)-coated gold electrode acts an efficient electrochemical biosensor for highly selective detection of human norovirus with a detection limit of 1.7 copies/mL, which is 3-fold lower than the reported methods. The developed electrochemical biosensor was successfully applied to detect human norovirus prepared by standard procedure from oyster, which suggests that the developed biosensor can be used as a very sensitive and selective point-of-care bioanalytical platform for the detection of human norovirus in various food samples.
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