期刊
BIOSENSORS & BIOELECTRONICS
卷 118, 期 -, 页码 195-203出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.07.052
关键词
Impedance cytometry; Dean flow fractionation; Leukocyte phenotyping; Diabetes mellitus
类别
资金
- NTU Research Scholarship
- Lee Kong Chian School of Medicine (LKCMedicine) Postdoctoral Fellowship
- Nanyang Technological University Start Up Grant
- National Research Foundation Singapore [CBRG-NIG (NMRC/BNIG/2038/2015)]
- Singapore Ministry of Education Academic Research Fund Tier 1 [RG37/15]
Circulating leukocytes comprise of approximately 1% of all blood cells and efficient enrichment of these cells from whole blood is critical for understanding cellular heterogeneity and biological significance in health and diseases. In this work, we report a novel microfluidic strategy for rapid (< 1 h) label-free leukocyte sorting and impedance-based profiling to determine cell activation in type 2 diabetes mellitus (T2DM) using whole blood. Leukocytes were first size-fractionated into different subtypes (neutrophils, monocytes, lymphocytes) using an inertial spiral sorter prior to single-cell impedance measurement in a microfluidic device with coplanar electrode design. Significant changes in membrane dielectric properties (size and opacity) were detected between healthy and activated leukocytes (TNF-alpha/LPS stimulated), during monocyte differentiation and among different monocyte subsets (classical, intermediate, non-classical). As proof-of-concept for diabetes testing, neutrophil/monocyte dielectric properties in T2DM subjects (n = 8) were quantified which were associated with cardiovascular risk factors including lipid levels, C-reactive protein (CRP) and vascular functions (LnRHI) (P < 0.05) were observed. Overall, these results clearly showed that T2DM subjects have pro-inflammatory leukocyte phenotypes and suggest leukocyte impedance signature as a novel surrogate biomarker for inflammation.
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