期刊
BRAIN STRUCTURE & FUNCTION
卷 223, 期 1, 页码 77-90出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00429-017-1470-7
关键词
Excitatory synapses; Dendritic shafts; Somatosensory cortex; Dendritic spines; Inhibitory synapses; Serial section three-dimensional reconstruction; Dual-beam electron microscopy; FIB/SEM
资金
- Spanish Ministerio de Economia y Competitividad [SAF 2015-66603-P]
- European Union Seventh Framework Programme (Human Brain Project) [604102]
- Centro de Investigacion en Red sobre Enfermedades Neurodegenerativas (CIBERNED, Spain) [CB06/05/0066]
- European Union Horizon 2020 research and innovation programme [720270]
- Spanish Ministerio de Economia y Competitividad (Cajal Blue Brain Project, Spanish partner of the Blue Brain Project initiative from EPFL)
Knowing the proportions of asymmetric (excitatory) and symmetric (inhibitory) synapses in the neuropil is critical for understanding the design of cortical circuits. We used focused ion beam milling and scanning electron microscopy (FIB/SEM) to obtain stacks of serial sections from the six layers of the juvenile rat (postnatal day 14) somatosensory cortex (hindlimb representation). We segmented in three-dimensions 6184 synaptic junctions and determined whether they were established on dendritic spines or dendritic shafts. Of all these synapses, 87-94% were asymmetric and 6-13% were symmetric. Asymmetric synapses were preferentially located on dendritic spines in all layers (80-91%) while symmetric synapses were mainly located on dendritic shafts (62-86%). Furthermore, we found that less than 6% of the dendritic spines establish more than one synapse. The vast majority of axospinous synapses were established on the spine head. Synapses on the spine neck were scarce, although they were more common when the dendritic spine established multiple synapses. This study provides a new large quantitative dataset that may contribute not only to the knowledge of the ultrastructure of the cortex, but also towards defining the connectivity patterns through all cortical layers.
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